Introduction Embryoscopy and Fetoscopy Applications Future Directions References

EMBRYOSCOPY AND FETOSCOPY

Embryoscopy and fetoscopy are sometimes referred to as embryofetoscopy.  Embryofetoscopy is capable of providing knowledge about embryonic development as well as provide a method for prenatal investigation of high-risk pregnancies for recurrent genetic disorders, perform fetal intravascular transfusions, and  allow access to the embryo circulation [1,2].  Embryoscopy refers to visualization of the embryo 5 to 8 weeks after conception, while fetoscopy deals with visualization of the fetus 8 weeks following gestation.  Both procedures are performed via insertion of a high-resolution fiber-optic scope transadbominally or transcervically, either into the extracelomic space (before 11 weeks after conception) thus allowing visualization of the embryo through an intact amnion membrane,  or into the amniotic cavity (after 11 weeks)  [1].  A typical endoscope can vary from 1.7mm to 3.5mm in diameter and 0 to 30° in lens angle and is passed using ultrasound guidance [1].  Zero degree endoscopes are also used with resolution up to 50,000 pixels [3] (Figure 1).

                           

            Figure 2:  Schematic diagram of an embryoscope inserted transcervically                                                                                                                                         and passed through the chorion into extraoelomic space [2]
Figure 1:  Embryoscope [3]

Once the scope reaches the opaque chorion, a rapid blunt thrust by the tip of the  endoscope has to be applied perpendicular to the chorionic membrane to allow penetration  into the extracoelomic space (Figure 2) [2].  This procedure has to be performed very  carefully to avoid areas of amnion and chorion juxtaposition as well as the placenta [2].   Consequently, phenotypical properties of the fetus such as head, face, dorsal and ventral  walls, umbilical cord and yolk sac can be observed.  Video recording through the scope  can also be done for more thorough examination at a later date.    

Because this technique becomes more difficult or impossible to perform, especially for penetration  of the chorion when the chorion and amnion are fused (around 10 weeks after  conception), the procedure has incurred modifications to allow for transabdominal  approach (Figure 3) to reduce infection, amniotic membrane rupture or cervical trauma.   Typically, local anesthesia with 1% xylocaine is given to the patient  [3].  A 16-gauge, double-barrel instrument sheath with 0.8mm fiber optic endoscope and 27-gauge needle  can be passed transabdominally, again using ultrasound guidance.  A 1-2mm skin incision is sometimes necessary when the trocar is more than 2mm in diameter [4].  The procedure typically lasts 5 minutes and has 95% success rate [2], although intraamniotic bleeding can result in impaired visibility 15% of the time [3], especially in an anteverted uterus, so gentle saline flushing of the exocoelomic space may be used [4].  Aliquots of blood can also be removed from umbilical vessels or chorionic plate blood vessels using a heparinized needle. In the 1990’s, thin-gague embryofetoscopy was introduced where an 18 or 19G needle is introduced into the amniotic cavilty and the endoscope is <0.9mm in diameter [3].  Today flexible endoscopes of  0.5 and 0.7 mm diameters are widely available, which can fit through 20-gauge and 18-gauge needles, as well as endoscopes with wider field of view and high clarity [5].


Figure 3:  Schematic  diagram of double-barrel needle
embryoscope inserted  transabdominally into  the amniotic
cavity  to perform fetal blood sampling  [2]