Single-Cell Isolation Array For Drug Screening and Drug Regimen Design

Introduction

Principles of Single-Cell Analysis
Device Fabrication and Design
Uses in Clinical and Lab Settings
Further Reading
References

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References

Literature

  • Svahn et al. “Single Cells or Large Populations?” Lab on a Chip, 2007, 7: 544-546
  • Navratil et al. “Microfluidic Devices for the Analysis of Single Cells: Leaving No Protein Uncounted” Sci. STKE, 2007, 388
  • Toner et al. “Blood-on-a-Chip” Annu. Rev. Biomed. Eng., 2005, 7: 77-103
  • McDonald et al. “Fabrication of microfluidic systems in poly(dimethylsiloxane)” Electrophoresis, 2000, 21:27-40
  • Whitesides et al. “Soft Lithography in Biology and Biochemistry” Annu. Rev. Biomed. Eng., 2001, 3:335–73
  • Wang et al. “High-density microfluidic arrays for cell cytotoxicity analysis” Lab on a Chip, 2007, 7: 740-745
  • Lee et al. “Dynamic single cell culture array” Lab on a Chip, 2006, 6:1445-1449
  • Di Carlo et al. “Dynamic Single-Cell Analysis for Quantitative Biology” Analytical Chemistry 2006, 78(23):7918-25.
  • White et al. “High-Throughput Screening in Drug Metabolism and Pharmacokinetic Support of Drug Discovery” Annu. Rev. Pharmacol. Toxicol. 2000, 40:133-157
  • Rettig et al. "Large-Scale Single-Cell Trapping and Imaging Using Microwell Arrays", Analytical Chemistry 2005, 77:5628

Image sources other than the above:

  • http://www.hku.hk/patho/facilities/images/cytometer-b.jpg
  • http://www.compucyte.com/images/lsc.jpg
  • http://www.paxit.com/imgs/automated_microscopy.jpg
  • http://faculty.washington.edu/afolch/FolchLabResearchProjects.htm
  • http://picasaweb.google.com/LiBachman/Nanovolume
  • http://www.coe.berkeley.edu/labnotes/0305/commuter.html

This site prepared using

by Jason Luo
Department of Biomedical Engineering
University of California, Irvine